Description Advanced BioMatrix offers PhotoCol®, a purified methacrylated type 1 bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness. The methacrylated type 1 collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. Kit 1 – PhotoCol® #5198-100MG Item Catalog Number Package Size Methacrylated Collagen #5198 100 mg PhotoCol® kits consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit. Kit 2 – PhotoCol®-IRG #5201-1KIT Item Catalog Number Package Size Storage Temperature Methacrylated Collagen #5198 100 mg 2-10°C 20 mM Acetic Acid #5079 50 mL 2-30°C Neutralization Solution #5205 10 mL Room Temperature Irgacure Photoinitiator #5200 100 mg 2-10°C A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm. Kit 3 – PhotoCol®-LAP #5270-1KIT Item Catalog Number Package Size Storage Temperature Methacrylated Collagen #5198 100 mg 2-10°C 20 mM Acetic Acid #5079 50 mL 2-30°C Neutralization Solution #5205 10 mL Room Temperature LAP Photoinitiator #5269 100 mg 2-8°C A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS which allows blue light photocrosslinking of the collagen at 405 nm. To sterile filter, resuspend and filter through a 0.2 micron button filter. Kit 4 – PhotoCol®-RUT #5271-1KIT Item Catalog Number Package Size Storage Temperature Methacrylated Collagen #5198 100 mg 2-10°C 20 mM Acetic Acid #5079 50 mL 2-30°C Neutralization Solution #5205 10 mL Room Temperature Ruthenium Photoinitiator #5246 100 mg Room Temperature Sodium Persulfate Photoinitiator #5247 500 mg Room Temperature A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1x PBS or cell culture media which allows visible light photocrosslinking of the collagen at 400-450 nm. We recommend using antibiotics with the photoinitiator, or filtering through a 0.2 micron button filter for cell culture work. Specifications Parameter, Testing, and Method Methacrylated Collagen #5198 Sterilization Method Filtration Extraction Method Acid – Telocollagen Form Lyophilized Powder Degree of Methacrylation >20% Package Size 100 mg Shelf Life Minimum of 6 months from date of receipt Shelf Life After Reconstitution 2 Months Collagen Purity – Silver Staining >99% Kinetic Gel Test (Minutes) <40 Gel Formation Tube Test (Minutes) < 40 Electrophoretic Pattern – Coomassie Blue Characteristic Sterility – USP modified No growth Endotoxin – LAL <10.0 EU/mL Source Bovine Hide Hydrogel Young’s Modulus E (Pa) Characteristic Disclaimer This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
