PureCol® EZ Gel, Bovine Collagen, Solution, 5 mg/mL

Description PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture. PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution. Specifications Parameter, Testing, and Method PureCol® EZ Gel Type I Collagen #5074 Sterilization Method Filtration Extraction Method Enzyme – atelocollagen Form Solution Package Size 35 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration – Biuret ~5 mg/mL Collagen Purity – Silver Staining >99% pH 6.9-7.4 Kinetic Gel Test (Minutes) <40 Gel Formation Tube Test (Minutes) <40 Fibrillogenesis (Absorbance Units) >0.5 Electrophoretic Pattern – Coomassie Blue Characteristic Sterility – USP modified No growth Endotoxin – LAL <1.0 EU/mL Osmolality (mOsmo H2O/kg) 300-360 Cell Attachment Assay Pass Source Bovine Hide Hydrogel Young’s Modulus E (Pa) Characteristic Medium Supplement DMEM/F-12 L-Glutamine Source Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) Product disclaimer This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Not available for shipment to Europe.